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. 2017 Dec 14;46(2):861–872. doi: 10.1093/nar/gkx1247

Figure 1.

Figure 1.

Activity of SsoTopA in bulk experiments. (A) For relaxation, the protein/pTZ18R DNA molar ratio used was 1:1 and the incubation time was as indicated. The reaction products were analyzed by one dimensional agarose gel electrophoresis. (B) Decatenation of kDNA was carried out by adding SsoTopA just before the incubation at the indicated temperature for 30 min in the presence of kDNA. The reactions were stopped and kDNA products were separated on a 2% agarose gel. OC indicates open circular DNA, Rel, the relaxed topoisomers and -SC the negatively supercoiled DNA for the pTZ18R plasmid (A) or the mCs (B).