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. 2017 Dec 14;46(2):861–872. doi: 10.1093/nar/gkx1247

Figure 2.

Figure 2.

Activity of SsoTopA at very high temperatures. (A) The DNA controls (lanes Ct°c) were incubated in the absence of SsoTopA for 8 min at the indicated temperatures. (B) The pTZ18R substrate was incubated at the temperature indicated in the presence of SsoTopA (at a molar ratio of 2:1). C75 corresponds to the DNA control incubated at 75°C for 8 min without SsoTopA. The reaction products were analyzed by one dimensional agarose gel electrophoresis: OC indicates open circular DNA, Rel, the relaxed topoisomers and -SC the negatively supercoiled DNA form. FI* and FI** correspond to unlinked DNA species and were apparently similar to highly unwound species first described by Parada and Marians (42).