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. 2017 Dec 14;46(2):861–872. doi: 10.1093/nar/gkx1247

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Reversible activity of SsoTopA. (A) Kinetics of DNA melting catalyzed by SsoTopA. The pTZ18R substrate relaxed by SsoTopA (molar ratio of 2:1) at 75°C was further incubated at 95°C in the presence of TopA for the time indicated above each corresponding lane. (B) Kinetics of DNA re-annealing catalyzed by TopA. The reaction mix (SsoTopA and resulting products obtained after 8 min at 95°C, panel (A) was further incubated at 75°C. The incubation times are indicated above each corresponding lane. The reaction products were analyzed by one dimensional agarose gel electrophoresis: OC indicates open circular (nicked) DNA, Rel, the relaxed topoisomers and -SC the negatively supercoiled DNA. FI* and FI** are the unlinked DNA species.