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Relaxation activity of SsoTopA on single molecule containing a permanent single-stranded DNA bubble. (A) Time-trace of the DNA extension variation at 35°C of the 2.2 kb DNA extended by a force of 0.42 pN and in the presence of 6 pM SsoTopA. (B) Histograms of the burst processivity of SsoTopA on a DNA containing a bubble. The number of supercoils removed was obtained from the extension variation using the calibration curve.
