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. 2017 Dec 14;46(2):730–747. doi: 10.1093/nar/gkx1240

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Global APA modulation is not detected following DSB induction. (A) Distribution of the CSs over different genome categories showed a highly significant enrichment for annotated protein-coding 3′UTRs. (B) As an additional indication of the precision of CS mapping by 3′-Seq, we searched for PAS signals (the canonical and its main variants) in ±100 nt with respect to the mapped CS. The PAS signals were significantly enriched at the correct location ∼20 nt upstream the CSs. (C) For each 3′UTR that showed a shift in CS usage upon NCS treatment, the usage of the proximal CS (relative to the usage of the other CSs in the same 3′UTR) was calculated. We call this relative usage Proximal Usage Index (PUI). A comparison of the distribution of PUIs between the NCS treated and control samples yielded no difference, indicating that there was neither global enhancement nor global reduction in usage of proximal CSs upon NCS treatment.