Fig. 4.

1950 MHz EMF exposure does not directly affect the processing and secretion of Aβ in 7w-PSML cells. (A) 7w-PSML cells were exposed to RF-EMF radiation (1950 MHz at 6 W/kg) for 2 h per day for 3 days. Secreted peptides in the culture medium were precipitated by TCA. APP and BACE1 in cell lysates, and sAPP (α or β) in precipitated samples, were detected by western blot analysis. β-actin was used as a loading control. (B) The relative band intensity of APP and BACE1 in cell lysates to the loading control was calculated by GelQuantNet software from the results of three independent experiments, including (A). Data are represented as mean ± S.E.M. from three independent experiments. P > 0.05 (non-significant, n.s). (C) Total 30 and 50 μg proteins of the TCA precipitated medium were separated by a 4–20% gradient precast Tris-Glycine SDS gel, and Aβ peptides were detected with anti-6E10 by western blot analysis.