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. 2017 Oct 6;59(1):18–26. doi: 10.1093/jrr/rrx045

Fig. 5.

Fig. 5.

1950 MHz EMF exposure has no significant effect on the APP protein expression and Aβ secretion in either HT22 or SH-SY5Y cells. (A–F) HT22 (A, B, C) and SH-SY5Y (D, E, F) cells were exposed to 1950 MHz at 6 W/kg for 2 h per day for 3 days. (A, D) Expression of APP and BACE1 in cell extracts of (A) HT22 and (D) SH-SY5Y was analyzed by western blot. β-actin was used as a loading control. (B, E) The relative band intensity of APP and BACE1 in cell lysates of (B) HT22 and (E) SH-SY5Y to the loading control was calculated by GelQuantNet software from the results of three independent experiments, including (A) and (D). Data are represented as mean ± S.E.M. from three independent experiments. P > 0.05 (non-significant, n.s). (C, F) Cell culture medium was precipitated with TCA, and secreted peptides were analyzed by a 4–20% gradient precast Tris-Glycine SDS gel. Secreted Aβ peptide was detected by western blot analysis with anti-6E10 in (C) HT22 and (F) SH-SY5Y cell media.