Figure 6.

NETs in aspirated thrombus from a patient with malignancy. (A) NETs immunostaining demonstrates both nuclear (arrowheads) and extensive extra-cellular signal (arrows). (B) Following incubation of adjacent tissue sections with DNase-1, immunostaining demonstrated absence of extracellular signal indicating the specificity of the antibody for NETs; only nuclear immunostaining of NETs was detected (arrow), as expected. Red = anti-histone H2A/H2B/DNA complexes (NETs); white bar indicates 100 microns.