Figure 4.

Effect of sharp triangle structural defects on REase reactivity. (A) The single-site resolution analysis of HhaI reactivity (see also Figure 3A) was repeated using sharp triangle variants with a defect in one of the three trapezoidal components (see the square hole in each diagram on the left). Variants were generated by omitting four specific staples (see detailed diagram at the bottom). Agarose gel electrophoresis was used to analyse HhaI reaction products of the three triangle variants (L, R and B). Results for the unmodified triangle (Figure 2B) are shown on top for comparison. (B) Diagrams showing the reactive sites in the defective sharp triangles, according to the analysis shown in A, and based on duplicate experiments (Supplementary Figure S16). ‘on’ (red), ‘int’ (pink), and ‘off’ (grey) labels are defined by the legend in Figure 3D.