Figure 5.

Ribosomal profiling confirms a predictive role for TIS efficiency in start codon utilization in the genome. The frequency of start codon utilization, the percentage of potential TIS motifs that were observed to act as translation initiation sites, in the 5′ leader sequences of three independent ribosomal profiling experiments. Each study used a unique small molecule drug to stall initiating ribosomes. A) Lactimidomycin - Lee et al, 2011, B) Harringtonine - Ingolia et al, 2011, C) Puromycin - Fritsch et al, 2012. P-values were calculated using Fisher's exact test. * denotes a P-value < 0.05; ** denotes a P-value < 0.001.