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. 2017 Dec 9;46(2):538–545. doi: 10.1093/nar/gkx1238

Figure 1.

Figure 1.

Workflow to study length-dependent amplification bias of RCA. (A) Equimolar ratios of single-stranded circular DNA templates of different length were pooled in one reaction tube. (B) These template mixes were amplified in the same tube by RCA by a strand displacement polymerase (e.g. Phi29 polymerase). (C) A restriction digest with nicking enzymes yielded monomeric copies of the circular templates of different length. (D) The relative length-dependent amplification efficiency was determined by denaturing PAGE (schematic).