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. 2017 Dec 4;46(2):804–822. doi: 10.1093/nar/gkx1205

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Fusing the CTD of p53 to GST renders GST a target for covalent PARylation. (A) In-vitro PARylation assay with 97 pmol GST, GST-p53_325–393 or GST-p53_359–387. In comparison, covalent PARylation of 5 pmol p53_WT is shown. (B) Thrombin cleavage assay with covalently PARylated GST-p53_325–393. Down-shifted bands are indicated by red brackets, bands of non-thrombin-treated samples by blue brackets. (C) Thrombin cleavage assay with covalently PARylated GST-p53_359–387.