Skip to main content
. 2017 Dec 8;46(2):972–984. doi: 10.1093/nar/gkx1213

Figure 3.

Figure 3.

The hCtc1(OB) disease mutant proteins are more stable than the WT protein. (A) SDS-PAGE gel showing the WT and the mutant R840W and V871M hCtc1(OB) purified proteins used in the experiments carried out for this study. (B) Normalized thermal denaturation curves derived from three independent DSC experiments for each of the WT and mutant R840W and V871M purified proteins shown in panel A. Detailed analysis of the data and Tm calculation is provided in Supplementary Figure S3. (C) Quantification of the Tm of the WT and the mutant R840W and V871M proteins using DSC. (D) Table of the melting temperatures of the WT and mutant hCtc1(OB).