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. 2017 Nov 14;46(2):609–620. doi: 10.1093/nar/gkx1110

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Nucleus is more compact in pht1Δ cells than in pht1⁺ cells. (A–C) pht1⁺ and pht1Δ cells expressing Rec10-GFP were induced to enter meiosis by nitrogen withdrawal. Cells undergoing horsetail movement were selected and their nucleus lengths were measured. (A and B) (Left panels) A maximum-intensity projection image of pht1⁺ (A) and pht1Δ(B) cells. The scale bars are 5 μm. (Right panels) Nuclei shown in the left panels are magnified and aligned. The scale bars are 5 μm. Note that these images are maximum-intensity projections and do not reflect decreased Rec10-GFP signal intensity in pht1Δ cells (Supplementary Figure S9B), which was revealed by summing signal intensities of all focal panels. (C) A box plot summarizing the observation of 96 (pht1⁺) and 83 (pht1Δ) nuclei. (D) The distance between ade1 and ade8 loci in pht1⁺ and pht1Δ horsetail nuclei was measured as described before (46). A box plot summarizing the observation of 47 (pht1⁺) and 49 (pht1Δ) nuclei is shown.