Table 2.
Diversity, frequency, and molecular features of canine-derived Giardia duodenalis isolates at the glutamate dehydrogenase locus. Castellón, Eastern Spain, 2014–2016. GenBank accession numbers are provided. Novel genotypes are shown underlined
| Assemblage | Sub-assemblage | No. isolates | Dog status | Reference sequence | Stretch (pb) | Single nucleotide polymorphism(s) | GenBank accession number |
|---|---|---|---|---|---|---|---|
| A | AII | 1 | Hunting | L40510 | 78–482 | A175R, A271R | MF285561 |
| 1 | Sheltered | L40510 | 78–482 | C198T | MF285562 | ||
| B | BIII | 1 | Shepherd | AF069059 | 54–455 | C87T, G93R, T95Y, C99Y, T147Y, G150R, T230Y, G277R, C309T | MF285563 |
| 1 | Sheltered | AF069059 | 54–455 | C87T, T138Y, T147Y, T219Y, T237Y, C309Y, C330Y, G354A, G372R, T382K, G406R, A414R, G444R | MF285564 | ||
| 1 | Sheltered | AF069059 | 44–455 | C87Y, C99Y, T147Y, G189R, C309T, G354R, G406R | MF285565 | ||
| BIV | 1 | Shepherd | L40508 | 80–481 | T183C, T387C, C396T, C423T | MF285566 | |
| 1 | Sheltered | L40508 | 109–496 | C133A, T464Y | MF285567 | ||
| C | – | 1 | Sheltered | U60984 | 78–490 | No | MF285568 |
| D | – | 1 | Sheltered | U60986 | 80–481 | No | MF285569 |
| – | 1 | Sheltered | U60986 | 80–481 | G225R, T429C, G441A, | MF285570 | |
| – | 2 | Breeding, Sheltered | U60986 | 80–481 | T240C | MF285571 | |
| – | 1 | Hunting | U60986 | 80–481 | T240C, C375T | MF285572 | |
| – | 1 | Sheltered | U60986 | 80–481 | T240Y, T429Y, G441R | MF285573 | |
| – | 3 | Sheltered | U60986 | 80–481 | T240C, T429C, G441A, T459A | MF285574 | |
| – | 1 | Sheltered | U60986 | 80–481 | C375T | MF285575 | |
| – | 2 | Hunting, Sheltered | U60986 | 80–481 | T429C, G441A | MF285576 | |
| – | 1 | Sheltered | U60986 | 80–481 | C216Y, T429C, G441A, C471Y | MF285577 |
K: A/T; R: A/G; Y: C/T