Table 1.
Primer sequences of genes used in qRT-PCR. Gene names and transcript IDs are also listed
| Gene | ID | Primer sequence | |
|---|---|---|---|
| A. scabra | |||
| XET25 | TRINITY_DN127707_c4_g25_i2 | Forward | CGACGCTTATCTCCAAACC |
| Reverse | GCCATGCCTTGCTCTATC | ||
| GDSL esterase | TRINITY_DN125263_c6_g4_i1 | Forward | CTTCACCAACGGCTACAA |
| Reverse | CAGCCCGAGTAGAAGTTTATC | ||
| Dirigent protein 5 | TRINITY_DN89062_c0_g1_i1 | Forward | GGACCATCACAGAAGAAAGTAG |
| Reverse | CCAGGTTGAAAGAGACATAGTAG | ||
| P5CR | TRINITY_DN120079_c1_g2_i1 | Forward | GGTAAGCGAGACAGGTAAAC |
| Reverse | GCGTCCCACGAAATGAA | ||
| Cytochrome P450 77A3 | TRINITY_DN133782_c0_g2_i3 | Forward | GATGGATGGACAAGCATCAT |
| Reverse | CAGCAGGTTATAGGTACACTTC | ||
| HMGB7 | TRINITY_DN119330_c0_g1_i2 | Forward | TGAAGAGGTGGAGGAAGAG |
| Reverse | CAGAAACTCTCACACAGAAGAG | ||
| DREB1A | TRINITY_DN125656_c0_g3_i2 | Forward | GCTGTGAGAGTTTCTGGTAAT |
| Reverse | AGCTCAGGTCGTTCTACATA | ||
| A. stolonifera | |||
| Glycine cleavage system H protein | TRINITY_DN88310_c1_g1_i3 | Forward | ACGGTCGCTGGATAGTATAA |
| Reverse | ACGTTCCTGCTCTACTATATCT | ||
| GAPDH A | TRINITY_DN108728_c4_g45_i1 | Forward | CATGGTTCCCTTGACGATT |
| Reverse | CCTATGTGATCGGTGTCAAC | ||
| Peroxidase 4 | TRINITY_DN101060_c1_g1_i1 | Forward | CGCTTGTCAGACTCTTCTTC |
| Reverse | TCCACGGATGGAGCTATT | ||
| Beta-glucosidase 3 | TRINITY_DN113597_c1_g1_i1 | Forward | GATGGGCAGCAGAACATAG |
| Reverse | GTGCTTGCAGAGAAGGTATAG | ||
| DIVARICATA | TRINITY_DN89810_c0_g3_i1 | Forward | GCCAACCCTCCTCATATAAA |
| Reverse | GTCCATAAACTACGGTAGGG | ||
| ACTIN | Internal reference | Forward | CCTTTTCCAGCCATCTTTCA |
| Reverse | GAGGTCCTTCCTGATATCCA | ||