Table 12.
The qRT-PCR validation of selected genes in RNA-seq data
| Gene | ID | Species | Log2FC in qPCR | Log2FC in RNA-seq |
|---|---|---|---|---|
| XET25 | TRINITY_DN127707_c4_g25_i2 | A. scabra | 4.57 | 6.60 |
| GDSL esterase | TRINITY_DN125263_c6_g4_i1 | A. scabra | 4.68 | 8.66 |
| Dirigent protein 5 | TRINITY_DN89062_c0_g1_i1 | A. scabra | 3.21 | 4.30 |
| P5CR | TRINITY_DN120079_c1_g2_i1 | A. scabra | 1.12 | 1.82 |
| Cytochrome P450 77A3 | TRINITY_DN133782_c0_g2_i3 | A. scabra | 3.68 | 6.45 |
| HMGB7 | TRINITY_DN119330_c0_g1_i2 | A. scabra | 2.82 | 5.13 |
| DREB1A | TRINITY_DN125656_c0_g3_i2 | A. scabra | 2.37 | 3.43 |
| Pearson’s correlation (A. scabra) | 0.95 | |||
| Glycine cleavage system H protein | TRINITY_DN88310_c1_g1_i3 | A. stolonifera | −1.98 | −2.49 |
| GAPDH A | TRINITY_DN108728_c4_g45_i1 | A. stolonifera | −2.31 | −3.09 |
| Peroxidase 4 | TRINITY_DN101060_c1_g1_i1 | A. stolonifera | −3.28 | −9.13 |
| Beta-glucosidase 3 | TRINITY_DN113597_c1_g1_i1 | A. stolonifera | −2.53 | −8.22 |
| DIVARICATA | TRINITY_DN89810_c0_g3_i1 | A. stolonifera | 3.27 | 5.44 |
| Pearson’s correlation (A. stolonifera) | 0.93 | |||