Table 1.
Sample | ka (1/Ms) | kd (1/s) | KD (kd/ka, M) |
---|---|---|---|
HA-F | 4.91 × 104 | 9.37 × 10−4 | 1.91 × 10−8 |
HA-HRA | 3.82 × 10 | 4.14 × 10−3 | 1.08 × 10−4 |
HA-HRB | 1.15 × 104 | 2.99 × 10−3 | 2.60 × 10−7 |
HA | 2.96 × 10 | 4.24 × 10−3 | 1.43 × 10−4 |
Note: kinetic and affinity data were determined by local fitting of the binding step of the individual sensorgrams to a 1:1 L binding model. ka: association rate constant; kd: dissociation rate constant; KD: the equilibrium dissociation constant (kd/ka)