Fig. 2.
The ability of EPS8 to influence the AML cells survival. a Western blot assay of EPS8 in six AML cell lines and an adriamycin-resistant AML cell line. b Cell survival assay in EPS8 shRNA-infected U937cells compared with NC shRNA-infected and parental cells on days 0 to 5. U937/NC and U937 cells nearly filled the well by 5th day. c Chemosensitivity of U937 cells transfected with shRNA2 after ADR treatment for 24 and 48 h. d U937 cells transfected with shRNA1, shRNA2 and NC shRNA were subjected to western blot analysis for EPS8, Akt/pAkt, Erk/p-Erk, p38 MAPK, p-GSK3β, p-cRaf, Caspase-9 and GAPDH expression. e Representative images of tumors extracted from nude mice following injection of 5 × 106 NC shRNA-infected cells and Eps8 shRNA-infected cells. The weights of the excised tumors