Fig. 5.

CP-EPS8-NLS potently suppresses human AML cell growth and increases chemosensitivity. a Flow cytometric analysis of Annexin V-FITC/PI–stained U937 cells treated with increasing concentrations of CP-EPS8-NLS for 24 h. b The effect of CP-EPS8-NLS on cell cycle: Following treatment of KG1α, U937, HL-60, TF1α, and THP-1 cells with CP-EPS8-NLS for 24 h and PI staining, cells were analyzed via flow cytometry and quantified. c Cells were cultured in the presence of daunorubicin (DNR; μmol/L), cytarabine (Ara-C; μmol/L), adriamycin (ADR; μmol/L) or CP-EPS8-NLS (μmol/L) alone or in combination at a fixed ratio. The combined treatment was highly effective in inducing cytotoxicity, as shown by CCK-8 assays at 24 h. The CI value for each data point was calculated with the appropriate software for dose effect analysis (CompuSyn). d Isobolograms were constructed using CompuSyn to further investigate the combination effects