Fig. 4.

Increased expression of ICAM-1 in MSCs facilitates cell adhesion and protects Jurkat cells from chemotherapeutic drugs. a Confocal top view and side view of Jurkat cells (prestained with MitoTracker Red) and GFP-labeled MSCs in a coculture system. Scale bar, 10 μm. b mRNA expression of adhesion molecules in MSCs cocultured with Jurkat cells. c Representative photos of Jurkat cells and MSCs in the coculture system after removal of the nonadhesive Jurkat cells. Scale bar, 50 μm. d The relative adhesion ratio was calculated as the ratio of the number of Jurkat cells adhered to MSCs in the anti-ICAM-1 treated group to that in the DMSO-treated group. e The death rate of Jurkat cells was examined by FACS. f A CCK-8 assay was used to assess Jurkat cell viability. The data above are presented as the mean ± S.E.M. of three independent experiments (*P < 0.05; **P < 0.01; t test)