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. 2018 Jan 22;11:11. doi: 10.1186/s13045-018-0554-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Cell-cell adhesion mediates mitochondria transfer from Jurkat cells to MSCs. Anti-ICAM-1 (20 mg/ml) was added to the coculture system with MTX for 48 h. a Confocal images showed that mitochondria transfer was inhibited by anti-ICAM-1. Scale bar, 10 μm. b The areas of red foci per field were calculated by ImageJ software. c Flow cytometry analysis of MitoTracker Red uptake by MSCs (GFP+ population) cocultured with MitoTracker Red-labeled Jurkat cells for 48 h. d The percentage of Red+ MSCs in each group was analyzed and graphed. The data above are presented as the mean ± S.E.M. of three independent experiments (*P < 0.05; **P < 0.01; t test)