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. 2018 Jan 22;9:1. doi: 10.1186/s13227-017-0089-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — sFRP-1 is necessary for ANE specification. Aa–e six3, foxq2, dkk3, nkx3.2, and sfrp-1/5 expression in mesenchyme blastula stage control embryos (24 hpf). Ag–k Embryos injected with sFRP-1 morpholino 1 show complete elimination of ANE markers. B qPCR measurements from three different cultures of embryos showing the downregulation of ANE regulatory genes at mesenchyme blastula stage (24hpf) in sFRP-1 morphants. The y axis shows the fold change in gene expression for sFRP-1 morphants versus controls. Scale bar = 20 μm. MO, morpholino