Figure 1.

Detection of EGFR mutations by ARMS-Plus and ddPCR in spiked cell mixtures. Mutant cell lines were spiked into a EGFR wild-type cell line at a ratio of 1 and 5%. The mutant abundance was then detected. EGFR (A) 19Del, (B) L858R, and (C) T790M mutations were stably detected by ARMS-Plus and ddPCR assays. Data are presented as the mean ± standard error of the mean. EGFR, epidermal growth factor receptor; ARMS, amplification refractory mutation system; ddPCR, droplet digital polymerase chain reaction; 19Del, exon 19 deletion.