Table II.

Sequence of primers and probes utilized for ARMS-Plus.

Site	Primer	Probe	Blocker
EGFR external control	Forward: 5′-TGGAGAGCATCCAGT-3′	5′FAM-ACATAGTCAGCAGTGACTT-3′MGB	/
	Reverse: 5′-TCTGGAAGTCCATCGACAT-3′
Del(1)	Forward: 5′-CCGTCGCTATCAAA-3′	5′FAM-AAGCCAACAAGGAAAT-3′MGB	/
	Reverse: 5′-ATGGACCCCCACAC-3′
Del(2)	Forward: 5′-GTCGCTATCAAGA-3′	5′FAM-AAGCCAACAAGGAAAT-3′MGB	/
	Reverse: 5′-GTCGCTATCAAGA-3′
L858R	Forward: 5′-AGATTTTGGGCG-3′	5′FAM-AACTGCTGGGTGCGGA-3′MGB	5′-TTTTTGGGCGGGCCAAAC-3′ phosphorthioated
	Reverse: 5′-TTTGCCTCCTTCTGC-3′
T790M(1)	Forward: 5′-CCGTGCAGCACATCAT-3′	5′FAM-CTCATGCCCTTCGGC-3′MGB	5′-GCTCATCACGCAGCTCA-3′ phosphorthioated
	Reverse: 5′-TGTCTTTGTGTTCCCG-3′
T790M(2)	Forward: 5′-CCGTGCAACACATCAT-3′	5′FAM-CTCATGCCCTTCGGC-3′MGB	5′-CAACTCATCACGCAGCT-3′ phosphorthioated
	Reverse: 5′-TGTCTTTGTGTTCCCG-3′

EGFR, epidermal growth factor receptor; ARMS, amplification refractory mutation system.