Figure 2. The MS risk allele rs4896153^T is associated with decreased AHI1 expression in naive CD4⁺ T cells and T cell receptor–stimulated CD4⁺ T cells.

(A) Naive CD4⁺ T cells were isolated from the peripheral blood of healthy subjects bearing risk (TT), protective (AA), or heterozygous (AT) genotypes for rs4896153, and AHI1 expression was measured by Taqman qPCR. The 2 major isoforms of AHI1 (B) isoform A (mRNA = NM_017651.4, NM_001134830.1, NM_001134831.1; protein = NP_001128303.1) and (C) isoform B (mRNA = NM_001134832.1; protein = NP_001128304.1) were measured in genotype individuals from (1A) by SYBR green qPCR. (D) Naive CD4⁺ T cells were stimulated with anti-CD3/CD28 for 0–48 hours, and AHI1 expression was measured by Taqman qPCR. Comparing the AA and TT genotypes, all data points are p < 0.01. Each dot represents an individual. Data are represented as mean ± SEM.