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. 2017 Dec 19;15(3):2889–2898. doi: 10.3892/ol.2017.7654

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Copyright: © Zou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Schematic illustration of the TMT-based quantitative whole proteomic/phosphoproteomic pipeline. Cells treated with ginsenoside Rg3 for different time were subjected to filter-assisted sample preparation-based sample preparation. 6-plex TMT labeling and bRP-HPLC were performed and collected fractions were concatenated. A proportion of these fractions were subjected to TiO₂ enrichment. Enriched phosphopeptides, along with unprocessed peptides were analyzed using an Orbitrap-equipped mass spectrometer. TMT, tandem mass tag; LC-MS/MS, liquid chromatography tandem mass spectrometry; bRP-HPLC, basic reversed phase high-performance liquid chromatography; TiO₂, titanium dioxide.