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Effects of the oleanolic acid derivative on ΔΨm. SMMC-7721 cells were incubated in the absence or presence of the oleanolic acid derivative (7, 9 or 11 µM) for the indicated times (12, 24 or 36 h). Cells were incubated with JC-1 for the detection of ΔΨm using fluorescence microscopic analysis. All images were captured at magnification ×100. ΔΨm, mitochondrial membrane potential; DMSO, dimethyl sulfoxide.
