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. 2017 Dec 21;15(3):3362–3368. doi: 10.3892/ol.2017.7684

Figure 2.

Figure 2.

Verification of pGL3-ENGFAP and PCA19-ENGFAP plasmids by gel electrophoresis, according to the size (bp) of excised fragments. (A) Excised fragments of pGL3-ENGFAP were detected by gel electrophoresis. Lanes 1–6, double digestion with MluI and XhoI; lane 7, GeneRuler™ DNA Ladder mix (10,000 bp); and lanes 8–13, double digestion with PvuI and PvuII. (B) Excised fragments of PCA19-ENGFAP were detected by gel electrophoresis. Lanes 1–6, double digestion with EcoRI and XbaI; lane 7, GeneRuler™ DNA Ladder Mix; and lanes 8–13, digestion with PstI. GFAP, glial fibrillary acidic protein.