Figure 3.

Increased PD-1 activates the PI3K/Akt1 pathway in CRL2631 cells. (A) Levels of phosphorylated PI3K and the phosphorylated Akt1 were assessed. n=3 independent experiments, and a representative blot is presented. (B) Quantification of the phosphorylated PI3K level relative to total PI3K. (C) Quantification of the phosphorylated Akt1 level relative to total Akt1. Data are presented as mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 with comparisons shown by lines. rPD-1, recombinant human programmed death ligand 1; si, small interfering; CTL, control; p, phosphorylated; PI3K, phosphoinositide 3-kinase; Akt1, protein kinase B; 1/6, 10 min; 1/2, 30 min.