Table I.
Primers used in the present study.
| Primer name | Primer sequence (5′-3′) |
|---|---|
| DACT2 qPCR | |
| Forward | CGGTCGGTTGATGAGACTACT |
| Reverse | CAGGGCTCTGTCAAGATCACC |
| CyclinD1 qPCR | |
| Forward | GCTGCGAAGTGGAAACCATC |
| Reverse | CCTCCTTCTGCACACATTTGAA |
| MMP7 qPCR | |
| Forward | GAGTGAGCTACAGTGGGAACA |
| Reverse | CTATGACGCGGGAGTTTAACAT |
| GAPDH qPCR | |
| Forward | ATGGGGAAGGTGAAGGTCG |
| Reverse | GGGGTCATTGATGGCAACAATA |
| DACT2 ORF | |
| Forward | CGGGATCCGCCGCTCGTGGGGTTCGGGA |
| Reverse | CGACGCGTACCATGGTCATGACCTTCA |
| β-catenin reporter (wide) | |
| Forward | CGCGTAACTGACAGATCAAAGGGGGTAAGATCAAAGGGGGTAGTCAACTC |
| Reverse | TCGAGAGTTGACTACCCCCTTTGATCTTACCCCCTTTGATCTGTCAGTTA |
| β-catenin reporter (mutant) | |
| Forward | CGCGTAACTGACAGATCCCCTTTTTTAAGATCCCCTTTTTTAGTCAACTC |
| Reverse | TCGAGAGTTGACTAAAAAAGGGGATCTTAAAAAAGGGGATCTGTCAGTTA |
| MSP primers | |
| Methylated forward | GCGCGTGTAGATTTCGTTTTTCGC |
| Methylated reverse | AACCCCACGAACGACGCCG |
| Unmethylated forward | TTGGGGTGTGTGTAGATTTTGTTTTTTGT |
| Unmethylated reverse | CCCAAACCCCACAAACAACACCA |
qPCR, quantitative polymerase chain reaction; DACT2, dishevelled-associated antagonist of β-catenin homolog 2; MMP7, matrix metalloprotease 7; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MSP, methylation-specific polymerase chain reaction.