Figure 1.
Prostratin induces cytotoxicity in breast cancer cells. (A) Dose titration of prostratin (0 to 200 µM) on MCF-7 cells; (filled squares) represent cells cultured under basal conditions; (open circles) represent cells cultured under high salt stimulating conditions (Δ0.05 M NaCl+0.1 ng/ml IL-17). (B) Dose titration was performed on the five breast cell lines, of which four (MCF-7, MDA-MB-231, BT-20, AU-565) are breast cancer lines and MCF-10 A is non-malignant breast epithelial cell line. Data were curve fit using the equation provided in methods section. IC50 and Cmax were obtained are presented above. One-way ANOVA analysis was performed for statistical analysis to determine the statistical significance of IC50 and Cmax for comparison between unstimulated and stimulated groups; significance taken at P<0.05, represented by astericks (*).