Extended data Table 1.
Data collection and refinement statistics
| K2p2.1 (TREK-1) (5VK5) | K2p2.1 (TREK-1): ML335 (5VKN) | K2p2.1 (TREK-1):ML402 (5VKP) | |
|---|---|---|---|
| Data collection | |||
| Space group | P212121 | P212121 | P212121 |
| Cell dimensions | |||
| a, b, c (Å) | 66.72/120.42/126.44 | 67.07/119.39/128.18 | 67.09/119.56/127.21 |
| α, β, γ (°) | 90.0/90.0/90.0 | 90.0/90.0/90.0 | 90.0/90.0/90.0 |
| Resolution (Å) | 87.2 – 3.10 (3.21 – 3.10) | 87.4 – 3.0 (3.11 – 3.0) | 87.1 – 2.8 (2.99 – 2.8) |
| Rsym (%) | 10.38 (>100%) | 23.7 (>100%) | 14.2 (>100%) |
| l/σ(l) | 13.35 (0.3) | 9.78 (0.66) | 11.13 (0.37) |
| CC1/2 | 0.999 (0.065) | 0.998 (0.171) | 0.999 (0.108) |
| Completeness (%) | 97.0 (100.0) | 97.0 (100.0) | 98.0 (100.0) |
| Redundancy | 6.0 (6.2) | 12.9 (13.3) | 12.8 (13.6) |
| Refinement | |||
| Resolution (Å) | 15.0 – 3.10 (3.21 – 3.10) | 15.0 – 3.0 (3.11 – 3.0) | 15.0 – 2.8 (2.99 – 2.8) |
| No. reflections | 18506 | 20686 | 25882 |
| Rwork/Rfree | 26.1/31.4 | 25.8/28.3 | 26.9/31.4 |
| No. atoms | |||
| Protein | 4289 | 4357 | 4328 |
| Ligand/ion | 74 | 200 | 174 |
| K+ | 6 | 6 | 6 |
| Cd2+ | 3 | 3 | 1 |
| Lipid | 65 | 191 | 167 |
| ML335 or ML402 | |||
| Water | 0 | 0 | 0 |
| B factors | |||
| Protein | 154.6 | 107.4 | 147.7 |
| Ligand/ion | 168.7 | 96.5 | 131.0 |
| Water | n/a | n/a | n/a |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.002 | 0.006 | 0.002 |
| Bond angles (°) | 0.480 | 0.685 | 0.483 |
a
Values in parentheses are for highest-resolution shell.
b
each dataset was derived from a single crystal