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. 2017 Dec 27;15(3):3109–3120. doi: 10.3892/ol.2017.7692

Table I.

Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers for the candidate miRNAs.

microRNA Name in mirBase Sequence in mirBase Length Modified primer sequence Primer length
hsa-miR-4455 >hsa-miR-4455 MIMAT0018977 AGGGUGUGUGUGUUUUU AGGGTGTGTGTGTTTTT 17 GCCAGGGTGTGTGTGTTTTT 20
hsa-miR-29a >hsa-miR-29a-3p MIMAT0000086 UAGCACCAUCUGAAAUCGGUUA TAGCACCATCTGAAATCGGTTA 22 TAGCACCATCTGAAATCGGTTA 22
hsa-miR-378b >hsa-miR-378b MIMAT0014999 ACUGGACUUGGAGGCAGAA ACTGGACTTGGAGGCAGAA 19 ACTGGACTTGGAGGCAGAA 19
hsa-miR-29b >hsa-miR-29b-3p MIMAT0000100 UAGCACCAUUUGAAAUCAGUGUU TAGCACCATTTGAAATCAGTGTT 23 GGTAGCACCATTTGAAATCAG 21
hsa-miR-205 >hsa-miR-205-5p MIMAT0000266 UCCUUCAUUCCACCGGAGUCUG TCCTTCATTCCACCGGAGTCTG 22 TCCTTCATTCCACCGGAGT 19
hsa-miR-100 >hsa-miR-100-5p MIMAT0000098 AACCCGUAGAUCCGAACUUGUG AACCCGTAGATCCGAACTTGTG 22 AACCCGTAGATCCGAACTTGTG 22
hsa-miR-7 >hsa-miR-7-5p MIMAT0000252 UGGAAGACUAGUGAUUUUGUUGU TGGAAGACTAGTGATTTTGTTGT 23 GCCTGGAAGACTAGTGATTTG 21
hsa-miR-375 >hsa-miR-375 MIMAT0000728 UUUGUUCGUUCGGCUCGCGUGA TTTGTTCGTTCGGCTCGCGTGA 22 TGTTCGTTCGGCTCGCGTGAA 21
hsa-miR-4299 >hsa-miR-4299 MIMAT0016851 GCUGGUGACAUGAGAGGC GCTGGTGACATGAGAGGC 18 TAGCTGGTGACATGAGAGG 19
hsa-miR-378h >hsa-miR-378h MIMAT0018984 ACUGGACUUGGUGUCAGAUGG ACTGGACTTGGTGTCAGATGG 21 ACTGGACTTGGTGTCAGATGG 21