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. 2017 Dec 28;15(3):3305–3312. doi: 10.3892/ol.2017.7708

Figure 3.

Figure 3.

Increased expression of c-Raf induced by downregulated cyclin M expression is a potential mechanism to increased chemoresistance. (A) Western blot analysis of c-Raf expression in c-Raf-silenced or non-c-Raf-silenced cells. β-actin was used as a loading control. (B) Cell viability (expressed as a percentage) of c-Raf-silenced or non-c-Raf-silenced subclones in response to 1 µg/ml gemcitabine. (C) Relative expression level of cyclin M and miR-433 in tissue samples from patients with gallbladder cancer (n=8). GR, gemcitabine-resistant; CDK10, cyclin-dependent kinase 10; miR, microRNA; siRNA, small interfering RNA; GBC, gallbladder cancer; CycM, cyclin M; Co, transfected with the plasmids which contained CDK10-ORF and CycM-ORF.