Skip to main content
. Author manuscript; available in PMC: 2018 Jan 23.
Published in final edited form as: Exp Hematol. 2016 Aug 31;44(12):1138–1155.e4. doi: 10.1016/j.exphem.2016.08.007

Figure 4. Effect of increasing concentrations of SB431542 on the expansion of progenitor (A) and precursor (B,C) cells in HEMA cultures of MNC from Cord Blood with (bottom panel) or without (top panel) Dex.

Figure 4

Figure 4

A) Cultured cells were collected at 0, 3, 6 and 10 Days of HEMA cultures of CB containing increasing concentrations of SB431542 (0, 10 and 26 μM) and their progenitor cell content evaluated as colony forming cells (CFC) in semisolid cultures. Results are presented as Mean of those obtained in two separate experiments, each one with a different donor. Statistical analysis was performed by Student’s t-test and the results are summarized in Fig. S2. See legend to Fig. 3 for further details.

B) Cells generated by MNC from CB cultured in HEMA in the presence of indicated concentrations of SB431542 (0, 10 or 26 μM) over time (0, 3, 6, 10, 12, 14 and 17 Days). Results are presented as fold increase with respect to Day 0 and are presented as Mean (± SEM) for two separated experiments each one with a different donor, performed in duplicate. Statistical analysis was performed by mixed models and Student’s t-tests and the results are summarized in Fig. S2.

C) Representative flow cytometric analyses for CD36 and CD235a of cells from CB cultured for 12 Days in the absence or presence of Dex and treated with SB431542 (0, 10 or 26 μM), as indicated. Results are representative of those observed in two experiments.