Table II.
Semi-automated quantification of IHC stainings of BON1 and HCT116 spheroids irradiated with 0, 2, 4, or 6 Gy.
| A, BON1 spheroids | |||
|---|---|---|---|
| IHC Stain | 0 Gy | 2 Gy | 4 Gy |
| Cyclin B1-positive staining (%) | 23.2±3.2 | 20.4±2.6 | 19.7±2.5 |
| Caspase-3-positive staining (%) | 7.7±1.2 | 11.3±1.8 | 20.1±3.4 |
| β1 galactosidase-positive staining (%) | 18.9±5.9 | 6.7±2.7 | 10.5±1.0 |
| B, HCT116 spheroids | |||
| IHC Stain | 0 Gy | 2 Gy | 6 Gy |
| Cyclin B1-positive staining (%) | 20.1±4.0 | 25.2±1.7 | 39.0±5.8 |
| Caspase-3-positive staining (%) | 10.8±1.3 | 16.2±0.7 | 40.9±2.5 |
| β1 galactosidase -positive staining (%) | 17.0±3.6 | 19.5±4.0 | 12.1±1.3 |
Quantifications are presented as percentage of positive staining per area. N>3, Data were presented as ± standard deviation. IHC, immunohistochemistry.