Figure 3.

Bergamottin treatment impairs the expression and secretion of MMP-9. Cells were treated with the indicated concentrations of bergamottin for 48 h and examined for MMP-9 expression. (A) Western blot analysis of MMP-9 protein. Blots are representative of three independent experiments. (B) Quantitative data from three independent experiments performed with three replicates are presented. (C) ELISA analysis of MMP-9 levels in the supernatants from treated cells. Quantitative data from three independent experiments performed with three replicates are presented. *P<0.05 vs. untreated cells; ^#P<0.05 vs. 2 µM bergamottin. MMP-9, matrix metalloproteinase-9.