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. 2018 Jan 6;7(1):e006320. doi: 10.1161/JAHA.117.006320

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© 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Acetylcholine esterase (AChE) stain revealing a reduction in Purkinje fibers of P18 Xirp2 ^−/− heart. Serial 10‐μm sections (sectioning from frontal/ventral to dorsal) of entire hearts from Xirp2 ^+/+ and Xirp2 ^−/− mice were histochemically stained for AChE activity (brown) to reveal the ventricular conduction system. Purkinje fibers were positively stained in representative sections of Xirp2 ^+/+ heart (a, section #252; b, #261; and c, #283), whereas comparable sections of Xirp2 ^−/− heart showed a drastically reduced in AChE activity (d, section #129; e, #139; and f, #149). Bar in e=1 mm for a through f. rv, right ventricle; lv, left ventricle; ivs, interventricular septum. (c′ and f′) higher magnification of the box regions in c and f, respectively. Bar in f’=0.2 mm for c′ and f′.