. 2016 Feb 5;16(1):17. doi: 10.1093/jisesa/iew003

Table 1.

Primer pairs used for qRT-PCR analysis of candidate reference genes and HSP70, a target gene

Gene symbol	Gene name	Accession number	Sequence (5′–3′)	Product length (bp)	Efficiency (%)	Tm (°C)	R²
β-ACT	Beta-Actin	KF018928.1	F: TCTTGGGAATGGAATCTTGC	254	98.10	60	0.994
β-ACT	Beta-Actin	KF018928.1	R: GGACAGAGAAGCCAAGATGG	254	98.10	60	0.994
18S	18S ribosomal	KF018922.1	F: ATTGACGGAAGGGCACC	157	106.09	60	0.998
18S	18S ribosomal	KF018922.1	R: CGCTCCACCAACTAAGAACG	157	106.09	60	0.998
28S	28S ribosomal	KC796354.1	F: GAGGTCCGTAGCGATTCTGA	105	99.25	60	0.996
28S	28S ribosomal	KC796354.1	R: GAGGGAAACTTCGGAGGGA	105	99.25	60	0.996
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	KP676380	F: ACTACTGTTCATGCAACCACCG	272	94.47	60	0.998
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	KP676380	R: GCTGCTTCCTTAACCTTATCCT	272	94.47	60	0.998
EF1α	Elongation factor 1 alpha	EU019874.1	F: GAAGCCTGGTATGGTTGTCGT	187	107.53	60	0.998
EF1α	Elongation factor 1 alpha	EU019874.1	R: GGGTGGGTTGTTCTTTGTG	187	107.53	60	0.998
TBP	TATA box binding protein	AGT79997.1	F: TGCTCCGAGTGAAGAAAAGG	171	103.78	60	0.994
TBP	TATA box binding protein	AGT79997.1	R: ACGGGCAAATGACTAGTGGA	171	103.78	60	0.994
RPL7	Ribosomal protein L7	KP676382	F: TGCCGGAGTCTGTACTCAA	255	94.70	60	0.999
RPL7	Ribosomal protein L7	KP676382	R: TCACACCACGAATACGCA	255	94.70	60	0.999
α-TUB	Alpha-Tubulin	KP676379	F: CCGTCAATTGTTCCACCCTG	195	91.33	60	0.998
α-TUB	Alpha-Tubulin	KP676379	R: CCAGATCCAGTACCACCTCC	195	91.33	60	0.998
HSP70	Heat shock protein 70 gene	KP676381	F: TCGCCTGTCTCAAGCCGAAAT	98	102.99	60	0.991
HSP70	Heat shock protein 70 gene	KP676381	R: GGTTCTTTGCCGCGATCTTG	98	102.99	60	0.991

F, forward primer; R, reverse primer; Tm, melting temperature; R², coefficient of correlation.