Table 1.

Primers used in cloning G. molesta RyR cDNA

Name	Primer namen	Primer sequence (5′–3′)	Description (length bp)
S1	F1	TTYCAYGTRACNCAYTGGTC	RT-PCR product S1 (824)
	R1	TGYTTYTCYTCGTGYTCCAT
S2	F2	TTCCGTGAACCTTGGCGAGA	RT-PCR product S2 (1,431)
	R2	GCAGGGATGTATCTTGTGGA
S3	F3	TGAGTTGCCGCTTCCTTCTT	RT-PCR product S3 (1,604)
	R3	CATCTGTTGGCGTTCCTTGA
S4	F4	GTAYACVAARGAYCARCCCAT	RT-PCR product S4 (1,482)
	R4	TCACCATCTCGCCAAGGTTCAC
S5	F5	MRDCCRCAYCARTGGGCTAG	RT-PCR product S5 (842)
	R5	GCRCCYTCVGCCATYTTCAT
S6	F6	CGAAAACTTGTTCCTGCCTC	RT-PCR product S6 (2,383)
	R6	ATACTCCGACAGCCGTGACA
S7	F7	CGHGARGCKGTBTCMGACTT	RT-PCR product S7 (854)
	R7	CKYTCVGCCATRTTYTGCAT
S8	F8	ACGGATTCAGCGACCCCATT	RT-PCR product S8 (1,394)
	R8	TGGTTGCCTTGAGTGGGAGT
S9	F9	TCWTAYTTRCCGTTCTGGTG	RT-PCR product S9 (1,586)
	R9	ATGTGGAGCAGCACCATCTC
S10	F10	ATMCAYGARCAAGARATGGA	RT-PCR product S10 (824)
	R10	CCTTCNARCATNGAHARCATCAT
S11	F11	AGTTGTCCAAGCACTCCTCG	RT-PCR product S11 (2,142)
	R11	CTCTTCGTGAGCCGCAAATG
S12	F12	TGGGACAARTTYGYRAAGAA	RT-PCR product S12 (716)
	R12	ATRAARCARTTGGAYTCCATGT
3′end	3′OF	CTGTGACGCATAATGGGAAGCA	RT-PCR product 3′RACE (1,143)
	3′IF	AAGAGGACGACGAGGTCAACAG
5′end	5′OR	AGAATCGCAGCACATCCCCACCG	RT-PCR product 5′RACE (977)
	5′IR	GGCTTGCGACATTACTGACCCTCC
RyR	RyR-F	GCTTCACCCGACGAGGCAGTGGAA	qRT-PCR (97)
	RyR-R	CTTGTGCTTGCTTCTTCGCTTGTTCTC
GAPDH	GF	GCCAGCTACGACGCCATCAAGCA	qRT-PCR (109)
	GR	CGCCGATGAAGTCAGAGGACACG
ASP-a	PaF	GAGCGAGCAGGATGATGTTT	diagnostic PCR for the presence of exon a
	PaR	AATTTTCTTTGCCGGTCTCG
ASA-a	AaF	TCCGAGACCGGTAAAGGCA	diagnostic PCR for the absence of exon a
	AaR	CCGTCGTGATGTGTCGTATG
AS-b	bF	TACAGCGGTAGTACAGAGTCG	diagnostic PCR for exon b
	bR	TCGTATCTGTGGGTTAGGAC
AS-c	cF	CACCGCGGGTCGACGGAAAGT	diagnostic PCR for exon c
	cR	TCGTATCTGTGGGTTAGGAC
ASP-d	PdF	GCCCAGTACAGCAGGTCAAG	diagnostic PCR for the presence of exon d
	PdR	AAGGCGTGGACTTGTAGCGA
ASA-d	AdF	AGTGTCACAG ACGAACCTCA	diagnostic PCR for the absence of exon d
	AdR	AAGGCGTGGACTTGTAGCGA
ASP-e	PeF	CAGATGTCGTGACGGATTCA	diagnostic PCR for the presence of exon e
	PeR	GGTGAGGAGGTCGTATGGGA
ASA-e	AeF	GTCTGGTGGC ACGGATTCAG	diagnostic PCR for the absence of exon e
	AeR	GGTGAGGAGGTCGTATGGGA
ASP-f	PfF	TACTCGTTCTATCCGCTGCT	diagnostic PCR for the presence of exon f
	PfR	AGCTCCGATTTTATGAGCCG
ASA-f	AfF	TCTTTACAGCAAACTGGGTT	diagnostic PCR for the absence of exon f
	AfR	CCTCTTGTCCGATGTTCTCT