Figure 2.

SAHA attenuates THP‐1 monocyte adhesion to HCAECs. HCAECs were plated in 6‐well plates the day before pretreatment with DMSO (vehicle) or SAHA (10 μmol/L) for 12 hours. Then, TNFα (10 ng/mL, final concentration) or vehicle (PBS) was added for an additional 6 hours. Then, THP‐1 monocytes (0.5 mL/well) were added for 30 minutes and then nonadherent monocytes were washed out. A, Representative images of monocyte adhesion assay. Cobblestone‐shaped cells are endothelial cells whereas small and round cells are adherent THP‐1 monocytes. B, Quantification of (A). Adherent THP‐1 cells were counted from 6 independent experiments. One‐way ANOVA with post‐hoc Bonferroni test; **P<0.01. SAHA indicates suberoylanilide hydroxamic acid; TNFα, tumor necrosis factor alpha.