Skip to main content
. 2017 Dec 4;6(12):e007159. doi: 10.1161/JAHA.117.007159

Figure 1.

Figure 1

Phosphofructokinase 2 (PFK‐2) content is reduced in diabetic mice without reduction of mRNA. A and B, Cardiac homogenates were analyzed from control and diabetic (4 months after streptozotocin treatment) mice for PFK‐2 and phosphorylated PFK‐2 (pPFK‐2; Ser483). C, PFK‐2 mRNA was measured by quantitative reverse transcription–polymerase chain reaction (RT‐qPCR). D, Metabolic enzymes related to PFK‐2 were analyzed by Western blot analysis. E, PFK‐2 was measured in cardiac homogenates of mice fed either a low‐fat control or a high‐fat diet for 21 weeks. Representative blots on the left and quantified on the right. F, Mice were fed either a low‐fat control or a high‐fat diet for 7 days. Protein homogenates were analyzed by a selected reaction monitoring technique. Data are presented as mean±SEM. NS indicates not significant (P=0.368); PDK4, pyruvate dehydrogenase kinase; PFKFB3, phospho fructokinase 2 isoform 3; and TIGAR, fructose‐2,6‐bisphosphatase. ***P<0.005, unpaired Student t test (n=4–6 for all groups).