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. 2017 Dec 2;6(12):e007441. doi: 10.1161/JAHA.117.007441

Figure 2.

Figure 2

A, The immunoblots of Nrf2 in nuclear protein when histone H3 was used as the internal reference. B, The immunoblots of phosphorylated MKK3 (p‐MKK3), MKK3, p‐MKK6, MKK6, p‐p38, p38, HO1 (heme oxygenase 1), and NQO1 (NADPH quinone oxidoreductase 1) in total protein when GAPDH was introduced as the internal reference. C through H, Columns in these panels indicate the relative expression level of Nrf2 (Nrf2/histon H3), phosphorylation level of MKK3 (p‐MKK3/MKK3), phosporylation level of MKK6 (p‐MKK6/MKK6), phosphorylation level of p38 MAPK, the relative expression levels of HO1 (HO1/GAPDH), and the relative expression level of NQO1 (NQO1/GAPDH), respectively (3 independent experiments were performed; *P<0.05; **P<0.01; ***P<0.001; # P>0.05).