Figure 7.

(A) Effects of the Nr5a2 reverse agonist, ML180. (A, left) Effects on genes associated with cell proliferation. (A, right) Mean reduction in proliferation, as indicated by the reduced expression of the marker Ki-67 by quantitative image analysis. (B) Representative image demonstrating reduction in Ki-67 expression. Original scale bars, 100 μM. (C) Effects of the β-catenin activity inhibitor iCRT3 on cell-cycle genes in granulosa cells in vitro harvested from gonadotropin-stimulated cKO mice. Means ± SE from cultures of granulosa cells from five mice per group. *P < 0.05, **P < 0.01, ***P < 0.001.