Figure 7. Combinatorial autoinhibitions of ankyrins and their potential regulations.

(A) Schematic diagram showing the combinatorial autoinhibition mechanisms of the three Ankyrin family members. ‘Sites-1,2,3’ are drawn according to our earlier study (Wang et al., 2014). R15-17 is assigned as ‘site-4’ based on the biochemical and structural analysis in this work and the concept that three to five ANK repeats can form a stable structural unit for target recognition, although no targets have been characterized to date. R18-24 is assigned as ‘site-5’ since it is autoinhibited by AI-c. The MBD binding sequences of the autoinhibitory segments or reported targets are shown in solid lines at corresponding positions of the bind sites. (B) Structural model of the autoinhibited full-length AnkB constructed with the three structures solved in the current study. The MBD is shown in surface representation. The three autoinhibitory segments are shown with the worm model. The unstructured loops are shown in grey dashed lines.