(
A) Phosphorimaging of the [
32P]-labeled RNAs generated from in vitro splicing assays carried out with WCE prepared from the given strains. (
B) Quantification of the results shown in (
A) depicting the accumulation of the fraction products that had completed the 1
st chemical step in splicing (lariat intron-3' exon and mRNA) over time for labeled extracts. (
C) Quantification of the results shown in (
A) depicting the accumulation of the fraction of second step product (mRNA) over time for labeled extracts. Descriptions of the each WCE corresponding to numbers listed in (
A–C) can be found in
Supplementary file 4.