Figure 7.

Effects of BC on ESR1 gene expression.

Notes: (A) The effect of BC, E₂, and ICI alone and in combination on ESR1 mRNA levels in T-47D breast cancer cells was determined by RT-qPCR. T-47D cells were treated in the presence or absence of 100 µM BC, 10 nM E₂, and/or 1 µM ICI for 24 hours. Results are shown as the mean ± SEM of at least three independent experiments with three replicates in each experiment. *p<0.05 (Kruskal–Wallis test followed by post hoc analysis using Mann–Whitney U test). (B) Calculation of PCR efficiencies. RT-qPCR efficiencies of reference ACTB and HPRT-1 genes and target gene (ESR1) were determined. Cq was plotted against the log amount of cDNA input. Amplification efficiencies were calculated according to the equation E = 10^(−1/slope).

Abbreviations: BC, black cohosh; SEM, standard error of the mean; ICI, ICI 182, 780; E₂, estradiol; RT-qPCR, reverse transcription quantitative real-time polymerase chain reaction; Cq, quantification cycle; DMSO, dimethyl sulfoxide; SYSR, SsoAdvanced SYBR Green Supermix.