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. 2018 Jan 18;10:1–11. doi: 10.2147/BCTT.S144865

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© 2018 Szmyd et al. This work is published and licensed by Dove Medical Press Limited

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Effects of BC on the cellular localization of ERα.

Notes: Treated T-47D cells were grown in 12-well growth plates, each well containing ~30,000 cells on coverslips. The cells were sustained for 2 days in whole media containing 10% FBS. They were then withdrawn from endogenous growth factors by culturing in DCC-FBS for 6 days. E₂, ICI, BC, and TAM were added in 2-day intervals alone or in combination for a period of 6 days. Cells were treated with Cy3 (red) and DAPI (blue) immunofluorescent stains and the cytolocalization of ERα protein was determined using confocal microscopy.

Abbreviations: BC, black cohosh; Cs, control; ERα, estrogen receptor-alpha; DCC, dextran-coated charcoal; FBS, fetal bovine serum; ICI, ICI 182, 780; TAM, Tamoxifen; E₂, estradiol.