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. 2018 Jan 24;38(2):313–323. doi: 10.1161/ATVBAHA.117.310418

Figure 1.

Figure 1.

MC1-R (melanocortin 1 receptor) deficiency accelerates atherosclerosis in Apoe−/− mice. A, Representative images of hematoxylin and eosin (H&E) staining of the aortic sinus of Apoe−/− and Apoe−/− Mc1re/e mice fed a high-fat diet (HFD) for 12 wk. Scale bar, 500 µm. B, Quantification of plaque area in aortic sinuses. C, D, Representative en face Sudan IV stainings and quantification of Sudan IV–positive lipid area in the aorta. E, Representative images of necrotic core areas, and α-SMA (α-smooth muscle actin) and Masson trichrome stainings in the aortic sinus. Scale bars, 200 µm (left) and 50 µm (middle, right). Necrotic areas are indicated with dashed lines in H&E-stained images. F, Quantification of acellular necrotic core areas as percentage of total plaque area. G, Quantification of α-SMA–positive area and plaque collagen content as percentage of total plaque area. n=8 to 10 mice per group in each graph. *P<0.05 and **P<0.01 vs Apoe−/− mice. Values are mean±SEM. Apoe indicates apolipoprotein.